| Name | Relationship | Address | Signature | Signature date | CIK |
|---|---|---|---|---|---|
| Burton Paul Frederick | Director | C/O CARDIO DIAGNOSTICS HOLDINGS INC., 311 W. SUPERIOR ST. SUITE 444, CHICAGO | /s/ Elisa Luqman as attorney-in-fact for Paul Burton | 03 Oct 2025 | 0002006956 |
| Type | Sym | Class | Transaction | Value $ | Shares | Change % | * Price $ | Shares After | Date | Underlying Class | Amount | Exercise Price | Ownership | Footnotes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| transaction | CDIO | Stock Options (Right to Buy) | Award | $6,252 | +1,559 | +46% | $4.01 | 4,953 | 30 Sep 2025 | Common Stock | 1,559 | $4.01 | Direct |