Rene Bernards - 30 Jun 2025 Form 4 Insider Report for LIXTE BIOTECHNOLOGY HOLDINGS, INC. (LIXT)

Role: Director
Signature: /s/ Rene Bernards
Issuer symbol: LIXT
Transactions as of: 30 Jun 2025
Net transactions value: $0
Form type: 4
Filing time: 08 Jul 2025, 21:33:02 UTC
Previous filing: 02 Apr 2025
SEC: View on sec.gov

Reporting Owners (1)

Name	Relationship	Address	Signature	Signature date	CIK
Bernards Rene	Director	KONINGSVAREN 37, ABCOUDE, NETHERLANDS	/s/ Rene Bernards	08 Jul 2025	0001939159

Derivative Securities (e.g., puts, calls, warrants, options, convertible securities)

Type	Sym	Class	Transaction	Value $	Shares	Change %	* Price $	Shares After	Date	Underlying Class	Amount	Exercise Price	Ownership	Footnotes
transaction	LIXT	Options to Purchase Common Stock	Award	$0	+10,000		$0.000000	10,000	30 Jun 2025	Common	10,000	$0.9050	Direct	F1
transaction	LIXT	Options to Purchase Common Stock	Award	$0	+10,662		$0.000000	10,662	30 Jun 2025	Common	10,662	$0.9050	Direct	F2
holding	LIXT	Options to Purchase Common Stock						8,045	30 Jun 2025	Common	8,045	$1.21	Direct
holding	LIXT	Options to Purchase Common Stock						4,166	30 Jun 2025	Common	4,166	$2.33	Direct
holding	LIXT	Options to Purchase Common Stock						5,304	30 Jun 2025	Common	5,304	$1.30	Direct
holding	LIXT	Options to Purchase Common Stock						10,000	30 Jun 2025	Common	10,000	$2.37	Direct
holding	LIXT	Options to Purchase Common Stock						4,149	30 Jun 2025	Common	4,149	$2.37	Direct

* An asterisk sign (*) next to the price indicates that the price is likely invalid.

Explanation of Responses:

Id	Content
F1	Effective June 30, 2025, the reporting person was granted stock options to purchase an aggregate of 10,000 shares vesting in eight equal installments on the last day of each subsequent quarter until fully vested with vesting to start on September 30, 2025.
F2	Effective June 30, 2025 the reporting person was granted stock options to purchase an aggregate of 10,662 shares vesting immediately. The grant was in lieu of the quarterly cash compensation due to the reporting person under the Board Compensation Plan.